Triplex-mediated analysis of cytosine methylation at CpA sites in DNA.
نویسندگان
چکیده
Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 5 شماره
صفحات -
تاریخ انتشار 2014